Modulating RssB activity: IraP, a novel regulator of S stability in Escherichia coli
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منابع مشابه
The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli.
In Escherichia coli, the adaptor protein SprE (RssB) controls the stability of the alternate sigma factor RpoS (sigma(38) and sigma(S)). When nutrients are abundant, SprE binds RpoS and delivers it to ClpXP for degradation, but when carbon sources are depleted, this process is inhibited. It also has been noted that overproduction of SprE is toxic. Here we show that null mutations in pcnB, encod...
متن کامل1 2 3 4 5 The response regulator SprE ( RssB ) modulates polyadenylation and mRNA stability in 6 Escherichia coli 7 8 Valerie
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 10 Department of Genetics, University of Georgia, Athens, Georgia 30605 11 12 13 14 Running title: SprE modulates RNA polyadenylation 15 16 17 18 *Corresponding author. Mailing address: Department of Molecular Biology, Princeton 19 University, Princeton, NJ 08544. Phone: (609) 258-5899. Fax: (609) 258-2957. E-mai...
متن کاملAnti-adaptors use distinct modes of binding to inhibit the RssB-dependent turnover of RpoS (σS) by ClpXP
In Escherichia coli, σ(S) is the master regulator of the general stress response. The level of σ(S) changes in response to multiple stress conditions and it is regulated at many levels including protein turnover. In the absence of stress, σ(S) is rapidly degraded by the AAA+ protease, ClpXP in a regulated manner that depends on the adaptor protein RssB. This two-component response regulator med...
متن کاملGrowth phase and (p)ppGpp control of IraD, a regulator of RpoS stability, in Escherichia coli.
The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its stabilization after DNA damage. In this study, we show by promoter fusions that ...
متن کاملIraL Is an RssB Anti-adaptor That Stabilizes RpoS during Logarithmic Phase Growth in Escherichia coli and Shigella
UNLABELLED RpoS (σ(S)), the general stress response sigma factor, directs the expression of genes under a variety of stressful conditions. Control of the cellular σ(S) concentration is critical for appropriately scaled σ(S)-dependent gene expression. One way to maintain appropriate levels of σ(S) is to regulate its stability. Indeed, σ(S) degradation is catalyzed by the ClpXP protease and the r...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 2006
ISSN: 0890-9369
DOI: 10.1101/gad.1400306